MircroarrayMicroarrays use reverse transcriptase to convert mRNA into complementary DNA (cDNA). The cDNA is then fluorescently labelled with probes. The labelled cDNA then can bind to corresponding sequences on a plate. If the cDNA binds, then a match has been found, indicating that the gene of interest was activated. The level of fluorescence will then indicate the level of expression [2].
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RNA-SeqmRNA is reverse transcribed into cDNA before being sequenced in short reads through bioinformatic methods. RNA seq provides several advantages such as you do not need to know what you are interested in before the experiment. However, it is more expensive and provides data that can be difficult to analyze [3].
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